Biosafety evaluation and quantitative determination of poly(hexamethylene biguanide) (PHMB) coated on cellulosic fabrics by Kubelka–Munk equation

It is difficult to directly determine the quantity of cationic finishing agents on surface cellulosic fabrics. Herein, we report a direct and feasible method by Kubelka–Munk equation quantify poly (hexamethylene biguanide) hydrochloride (PHMB) adsorbed onto cotton fabrics based principle formation stable blue dye between PHMB bromophenol sodium (BPB). The adsorption was first investigated maximum found be around 8 mg per gram fabric. After being dyed with BPB, colour strength shows positive correlation at low concentrations (< 2400 mg/L). A linear relationship high (C(PHMB) = (K/S—0.7411)/3.4517, R2 0.9983) thus established concentration. However, this should fulfill four requirements for quantifying PHMB: (1) distribution fabric in form monolayer content less than 5.3 mg/g; (2) an excess BPB dyebath applied; (3) dyeing come equilibrium; (4) evenly dyed. Moreover, methyl thiazolyl tetrazolium (MTT) assay results indicate that coated can cause cytotoxicity not exceed 4.62 (0.462% w/w) purpose biosafety. sample considered non-cytotoxic if K/S value lower 13.2 after BPB.